Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free
    See all Hormone sensitive lipase/HSL primary antibodies

  • Description

    Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects HSL phosphorylated on serine 853.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247849 is the carrier-free version of ab109400 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247849 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Hormone sensitive lipase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)
    Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)
    All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

    Lane 1 : Human skeletal muscle lysates
    Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 117 kDa
    Observed band size: 83 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109400, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)
    Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)
    All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 117 kDa
    Observed band size: 83 kDa why is the actual band size different from the predicted?



    This data was developed using ab109400, the same antibody clone in a different buffer formulation.

     

  • Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)
    Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (ab247849)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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