Anti-hnRNP H antibody (ab154894)
Key features and details
- Rabbit polyclonal to hnRNP H
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-hnRNP H antibody
See all hnRNP H primary antibodies -
Description
Rabbit polyclonal to hnRNP H -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 50-289 of Human hnRNP H (Uniprot ID P31943)
-
Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Mouse brain lysates. Jurkat, Raji and NCI-H929 cells IHC-P: NCIN87 xenograft tissue. ICC/IF: HeLa and A431 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-hnRNP H antibody (ab154894)All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)Immunocytochemistry/immunofluorescence analysis of A431 cells labelling hnRNP with ab154894 at 1/500 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/1000 dilution (red).
-
Western blot - Anti-hnRNP H antibody (ab154894)All lanes : Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1 : Jurkat cells
Lane 2 : Raji cells
Lane 3 : NCI-H2929 cells
Predicted band size: 49 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling hnRNP with ab154894 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/10000 dilution (red).
-
Western blot - Anti-hnRNP H antibody (ab154894)Anti-hnRNP H antibody (ab154894) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 49 kDa
10% SDS PAGE
