Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23054-108] to HNF1 alpha
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HNF1 alpha antibody [EPR23054-108]
    See all HNF1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR23054-108] to HNF1 alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Caco-2 and Hepa1-6 whole cell lysate; Mouse liver lysate; Rat liver lysate. IHC-P: Human liver and colon tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Western blot - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    All lanes : Anti-HNF1 alpha antibody [EPR23054-108] (ab272693) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 81 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: 3 minutes.

  • Western blot - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Western blot - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    All lanes : Anti-HNF1 alpha antibody [EPR23054-108] (ab272693) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 81 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).

    Negative control: HeLa (PMID: 10677375). 

    Exposure time: Lanes 1-3: 10 seconds; Lane 4: 3 minutes.

  • Flow Cytometry - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Flow Cytometry - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling HNF1 alpha with ab272693 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 10677375).

  • Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 and HeLa cells labelling HNF1 alpha with ab272693 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HepG2 cells. 100% methanol fixation is recommended. Negative control: HeLa (PMID: 10677375). ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF1 alpha with ab272693 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon. The section was incubated with ab272693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF1 alpha with ab272693 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver. The section was incubated with ab272693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)
    Anti-HNF1 alpha antibody [EPR23054-108] (ab272693)

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