Anti-HNF1 alpha antibody (ab96777)
Key features and details
- Rabbit polyclonal to HNF1 alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HNF1 alpha antibody
See all HNF1 alpha primary antibodies -
Description
Rabbit polyclonal to HNF1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
-
Immunogen
Recombinant fragment corresponding to Human HNF1 alpha aa 35-238.
Database link: P20823 -
Positive control
- WB: HepG2 whole cell lysate IHC-P: A549 Xenograft
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-HNF1 alpha antibody (ab96777)Anti-HNF1 alpha antibody (ab96777) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?7.5% SDS PAGE
Blocking: 5% non-fat milk in TBST, room temperature, 60 minutes.
Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).
Negative control: HeLa (PMID: 12488960). Also, 293T and A431.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (ab96777)ab96777, at a 1/100 dilution, staining HNF1 alpha in paraffin embedded A549 Xenograft by Immunohistochemistry.
-
Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody (ab96777)ab96777 staining HNF1 alpha in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/1000) at 4°C overnight and stained green, alpha tubulin stained red and Hoecsht 33342 stained blue.
Permeabilization: 0.1% Triton X-100 in PBS, 4 minutes.
Blocking: 2.5% BSA/PBS for 30 minutes.
Secondary antibody: Rabbit IgG antibody (Alexa Fluor 488), 1:2000 (keep from light), room temperature for 1 hour.
