Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)
![Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)](https://www.abcam.com/ps/products/242/ab242140/Images/ab242140-366149-anti-hnf1a-antibody-epr23054-142-western-blot-human-caco2.jpg "Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23054-142] to HNF1 alpha
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-HNF1 alpha antibody [EPR23054-142]
See all HNF1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR23054-142] to HNF1 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa and Caco-2 lysates. IHC-P: Human pancreas and Human liver tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23054-142 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution + Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID:31145732). Exposure time: 6 seconds
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![Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)](https://www.abcam.com/ps/products/242/ab242140/Images/ab242140-366148-anti-hnf1a-antibody-epr23054-142-western-blot-human-hepg2.jpg)
Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)All lanes : Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).
Negative control: HeLa (PMID: 12488960)
Exposure time: 5.5 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)](https://www.abcam.com/ps/products/242/ab242140/Images/ab242140-2-anti-hnf1a-antibody-epr23054-142-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF1A with ab242140 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver (PMID: 26169608) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)](https://www.abcam.com/ps/products/242/ab242140/Images/ab242140-1-anti-hnf1a-antibody-epr23054-142-immunohistochemistry-pancreas-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HNF1A with ab242140 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas (PMID: 25793983) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)](https://www.abcam.com/ps/products/242/ab242140/Images/ab242140-5-benefits-of-recombinant-antibodies.png)
Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)
