Anti-HMGB2 antibody (ab67282)
Key features and details
- Rabbit polyclonal to HMGB2
- Suitable for: ICC, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HMGB2 antibody
See all HMGB2 primary antibodies -
Description
Rabbit polyclonal to HMGB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatHumanIP HumanWB Mouse
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Immunogen
Synthetic peptide:
KGKSEAGKKGPGRPTGS
conjugated to KLH, corresponding to amino acids 165-181 of Human HMGB2 -
Positive control
- WB: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-HMGB2 antibody (ab67282)
Immunocytochemistry analysis of HUVEC cells using Anti-HMGB2 antibody (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).
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Western blot - Anti-HMGB2 antibody (ab67282)All lanes : Anti-HMGB2 antibody (ab67282) at 2 mg/ml
Lane 1 : NIH3T3
Lane 2 : Rat1
Lane 3 : RINSH
Lane 4 : PC12
Lane 5 : K562
Lane 6 : HEK-293T
Lane 7 : HeLa nuclear
Lane 8 : HeLa
Predicted band size: 24 kDa
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Immunocytochemistry - Anti-HMGB2 antibody (ab67282)Nuclear localization of HMGB2 in PC12 cells.
A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 µg/mL
B. DAPI staining
C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2 -
Immunoprecipitation - Anti-HMGB2 antibody (ab67282)Lanes
1: 5 µg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
2: Negative control – without cell lysate
3: Negative control – without antibody
4: Positive control – Extract of HEK-293T direct labeling
