Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)
![Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)](https://www.abcam.com/ps/products/248/ab248902/Images/ab248902-405386-anti-hla-drb4-antibody-epr7182-bsa-and-azide-free-western-blot.jpg "Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7182] to HLA-DRB4 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free
See all HLA-DRB4 primary antibodies -
Description
Rabbit monoclonal [EPR7182] to HLA-DRB4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248902 is the carrier-free version of ab140612. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248902 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7182 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)Anti-HLA-DRB4 antibody [EPR7182] (ab140612) at 1/1000 dilution + Raji cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaThis data was developed using ab140612, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)](https://www.abcam.com/ps/products/248/ab248902/Images/ab248902-2-anti-hla-drb4-antibody-epr7182-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)This data was developed using ab140612, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labelling HLA-DRB4 using ab140612 at 1/100 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)](https://www.abcam.com/ps/products/248/ab248902/Images/ab248902-1-anti-hla-drb4-antibody-epr7182-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)This data was developed using ab140612, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling HLA-DRB4 using ab140612 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)](https://www.abcam.com/ps/products/248/ab248902/Images/ab248902-4-benefits-of-recombinant-antibodies.png)
Anti-HLA-DRB4 antibody [EPR7182] - BSA and Azide free (ab248902)