Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [JS76] to HLA DRB1 + HLA-DPB1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free

  • Description

    Mouse monoclonal [JS76] to HLA DRB1 + HLA-DPB1 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Ramos whole cell lysate. IHC-P: Human tonsil tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252792 is the carrier-free version of ab241560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA DRB1 + HLA-DPB1 with ab241560 at 0.798µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab241560 for 30 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241560).

  • Western blot - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)
    Western blot - Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)
    All lanes : Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] (ab241560) at 0.399 µg/ml

    Lane 1 : Ramos (Human Burkitt’s lymphoma B lymphocyte) lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 114 seconds.

    Negative control: Hela (PMID: 20959457).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20959457).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241560).

  • Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)
    Anti-HLA DRB1 + HLA-DPB1 antibody [JS76] - BSA and Azide free (ab252792)

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