Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)

Key features and details

  • Mouse monoclonal [LN-3] to HLA Class II DRB1
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [LN-3]
    See all HLA Class II DRB1 primary antibodies

  • Description

    Mouse monoclonal [LN-3] to HLA Class II DRB1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class II DRB1. (Activated human peripheral blood mononuclear cells).

  • Positive control

    • IHC-P: Human histiocytoma and tonsil tissues. WB: Ramos and human spleen cells Flow Cyt: Raji cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from Bioreactor Concentrate by Protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    LN-3

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Western blot - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    All lanes : Anti-HLA Class II DRB1 antibody [LN-3] (ab233877) at 2 µg/ml

    Lane 1 : Ramos cells (human Burkitt's lymphoma cell line)
    Lane 2 : Human spleen lysate

    Predicted band size: 29 kDa

  • Western blot - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Western blot - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Anti-HLA Class II DRB1 antibody [LN-3] (ab233877) at 2 µg/ml + Ramos cells (human Burkitt's lymphoma cell line)

    Predicted band size: 29 kDa

  • Flow Cytometry - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Flow Cytometry - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)

    Flow cytometry analysis of Raji cells (human Burkitt's lymphoma cell line) stained with ab233877 in 2µg/million cells (blue) and an isotype control (red)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)

    Formalin-fixed, paraffin-embedded human histiocytoma tissue stained for HLA Class II DRB1 using ab233877 at 0.5 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [LN-3] (ab233877)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA Class II DRB1 using ab233877 at 0.5 μg/ml in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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