Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects Histone H3 and Histone H3.3 when phosphorylated on Serine 28. It does not detect H3.3 when phosphorylated on Serine 31.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247463 is the carrier-free version of ab76312 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247463 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP1874Y

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)
    Western blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)
    All lanes : Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312) at 1/5000 dilution

    Lane 1 : HeLa cell lysates untreated
    Lane 2 : HeLa cell lysates treated with FBS + calyculin A

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76312, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)
    Dot Blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)
    This data was developed using ab76312, the same antibody clone in a different buffer formulation.Dot blot performed using ab76312 at a dilution of 1/100. Lane 1 - Unmodified H3 peptide. Lane 2 - H3S28ph peptide. Lane 3 - H3.3S28ph peptide. Lane 4 - H3.3S31ph peptide. A HRP-conjugated goat anti-rabbit (H+L) was used as the secondary antibody at a dilution of 1/1000. The exposure time was 3 minutes and the dilution and blocking buffer used were 5% NFDM/TBST.
  • Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)
    Anti-Histone H3 (phospho S28) antibody [EP1874Y] - BSA and Azide free (ab247463)

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