Anti-Histone H3 (phospho S10) antibody (ab183626)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho S10)
- Suitable for: ChIP, IP, IHC - Wholemount, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho S10) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho S10) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanICC/IF HumanIHC - Wmt ZebrafishIHC-P HumanIP HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human Histone H3 (phospho S10). The exact sequence is proprietary. Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser10.
Database link: P68431 -
Positive control
- U2OS (100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr); RFP-tag transfected HeLa cells; Mitotic HeLa cells; Human gastric carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (phospho S10) antibody (ab183626)
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3 (phospho S10) antibody (ab183626). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1 : U2OS whole cell lysate (untreated)
Lane 2 : U2OS whole cell lysate (serum deprivation for 48hr)
Lane 3 : U2OS whole cell lysate (100ng/ml Nocodazole treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)Confocal immunofluorescent analysis of mitotic HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/2500 . Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S10) with ab183626 at 1/500.
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Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (ab183626)ab183626 immunoprecipitating Histone H3 in HeLa whole cell lysate.
Lane (-): Untreated HeLa whole cell lysate (30 µg)
Lane (+): Treated HeLa whole cell lysate (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ab183626 diluted at 1:2000.
For western blotting, HRP-conjugated anti-rabbit IgG was used as a secondary antibody.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/500 dilution
Lane 1 : Rat2 whole cell lysate
Lane 2 : Rat2 whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunofluorescent analysis of RFP-tag transfected HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/500 . Blue: Hoechst 33342 staining.
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Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)All lanes : Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1 : 4T1 whole cell lysate
Lane 2 : 4T1 whole cell lysate (100ng/ml Nocodazole treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
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IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
