Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM140] to Histone H3 (mono methyl K4)
Suitable for: ICC/IF, ELISA, WB, ChIP
Reacts with: Human, Recombinant fragment

Product name

Anti-Histone H3 (mono methyl K4) antibody [RM140]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [RM140] to Histone H3 (mono methyl K4)
Host species

Rabbit
Specificity

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

Tested Applications & Species

Application	Species
ChIP	Human
ICC/IF	Human
WB	Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide corresponding to Histone H3 (mono methyl K4).
Positive control
- Acid extracts of HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Purification notes

ab205256 was purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM140
Isotype

IgG
Research areas

ChIP - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

ChIP performed on HeLa cells using ab205256 (5ug). Real-time PCR was performed using primers specific to the gene indicated.
Western blot - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

All lanes : Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256) at 0.5 µg/ml

Lane 1 : Recombinant histone H3.3
Lane 2 : Acid extracts of HeLa cells

Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with sodium butyrate, labelling Histone H3 (mono methyl K4) with ab205256 at 1 – 2 µg/mL (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ELISA - Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

ab205256 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Anti-Histone H3 (mono methyl K4) antibody [RM140] (ab205256)

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