Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [ERP16597] to Histone H3 (mono methyl K4) - ChIP Grade
  • Suitable for: PepArr, WB, ICC/IF, IHC-P, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [ERP16597] to Histone H3 (mono methyl K4) - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 whole cell lysates. IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells. ChIP: Chromatin from HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    ERP16597

  • Isotype

    IgG

  • Research areas

Images

  • ChIP - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    ChIP - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min.

    The ChIP was performed with 25 µg of chromatin, 2 µg of ab176877 (red), or 2 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (Alexa Fluor® 594 goat anti-mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab176877 at 1/1000 dilution followed by ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor® 488; IgG H&L) at 1/400 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Western blot - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    All lanes : Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Peptide Array - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    ab176877 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP).  Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (mono methyl K4) with ab176877 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of colon tissue is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)
    Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade (ab176877)

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