Anti-Histone H3 (mono methyl K36) antibody (ab272157)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K36)
- Suitable for: ICC/IF, WB, Dot blot
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K36) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K36) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Dot blotmore details -
Species reactivity
Reacts with: Human, Caenorhabditis elegans
Predicted to work with: Rat, Chicken, Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (mono methyl K36).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo lysate. HeLa histone preps. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (mono methyl K36) antibody (ab272157)Anti-Histone H3 (mono methyl K36) antibody (ab272157) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) histone preps at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K36) antibody (ab272157)Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl L36) with ab272157 at 1/100 dilution for 1 hour at room temperature, followed by Dylight 488 secondary antibody at 1/10000 for 45 minutes at room temperature. Staining of Histone H3 (mono methyl L36) is green. Nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
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Western blot - Anti-Histone H3 (mono methyl K36) antibody (ab272157)Anti-Histone H3 (mono methyl K36) antibody (ab272157) at 1/1000 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Dot Blot - Anti-Histone H3 (mono methyl K36) antibody (ab272157)Dot Blot of ab272157.
Lane 1: K36.
Lane 2: K36Me1.
Lane 3: K36Me2.
Lane 4: K36Me3.
Lane 5: K36ac.
Load: 0.3, 0.6, 1, 3, 6, and 10 picomoles of peptide. Primary antibody ab272157 at 0.5 µg/ml for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.