Anti-Histone H3 (di methyl K36) antibody (ab272158)
Key features and details
- Rabbit polyclonal to Histone H3 (di methyl K36)
- Suitable for: ICC/IF, ChIP, WB, Dot blot
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (di methyl K36) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (di methyl K36) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ChIP, WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans
Predicted to work with: Rat, Chicken, Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (di methyl K36).
Database link: Q71DI3 -
Positive control
- WB: C.elegans embryo lysate. HeLa histone preps ICC/IF: Neuro-2a cells. ChIP: Chromatin prepared from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (di methyl K36) antibody (ab272158)
Chromatin Immunoprecipitation with ab272158. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 µg of ab272158 and 20 µl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution + HeLa histone preps at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K36) antibody (ab272158)Immunofluorescence of 0.5% PFA-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling Histone H3 (di methyl K36) with ab272158 at 1/100 dilution for 1 hour at room temperature, followred by FITC secondary antibody at 1/10000 for 45 minutes at room temperature. Histone H3 (di methyl K36) staining is green. Nuclei were counterstained with DAPI (blue).
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Western blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)Anti-Histone H3 (di methyl K36) antibody (ab272158) at 1/500 dilution + C.elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Dot Blot - Anti-Histone H3 (di methyl K36) antibody (ab272158)Dot Blot of ab272158.
Lane 1: K36.
Lane 2: K36Me1.
Lane 3: K36Me2.
Lane 4: K36Me3.
Lane 5: K36Ac.
Load: 1, 3, 6, and 10 picomoles of peptide. Primary antibody ab272158 at 1/1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.