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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM171] to Histone H3 (di methyl K23)
  • Suitable for: WB, ELISA
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-Histone H3 (di methyl K23) antibody [RM171]
    See all Histone H3 primary antibodies
  • Description

    Rabbit monoclonal [RM171] to Histone H3 (di methyl K23)
  • Host species

    Rabbit
  • Specificity

    ab214654 reacts to Histone H3 dimethylated at Lysine 23. There is no cross reactivity with nonmodified Lysine 23, monomethylated Lysine 23 (K23me1), or other methylations in histone H3.
  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 (di methyl K23).
    Database link: P68431

  • Positive control

    • Acid extracts of HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab214654 was purified from an animal origin-free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM171
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated

Images

  • Western blot - Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)
    Western blot - Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)
    All lanes : Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654) at 0.5 µg/ml

    Lane 1 : Recombinant histone H3.3
    Lane 2 : Acid extracts of HeLa cells
  • ELISA - Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)
    ELISA - Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)

    ab214654 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.

  • Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)
    Anti-Histone H3 (di methyl K23) antibody [RM171] (ab214654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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