Anti-Histone H2B (acetyl K15) antibody (ab240891)
Key features and details
- Rabbit polyclonal to Histone H2B (acetyl K15)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H2B (acetyl K15) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (acetyl K15) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Histone H2B (acetyl K15). The exact sequence is proprietary.
Database link: P62807 -
Positive control
- WB: HeLa, K562 and HEK-293, treated with with 30mM sodium butyrate for 4hr, whole cell lysates. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). IP: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Histone H2B (acetyl K15) antibody (ab240891)All lanes : Anti-Histone H2B (acetyl K15) antibody (ab240891) at 1/1000 dilution
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 4 : Hela whole cell lysate, untreated (-)
Lane 5 : K562 whole cell lysate, untreated (-)
Lane 6 : A549 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 14 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody (ab240891)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H2B (acetyl K15) using ab240891 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was an Alexa-Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
-
Immunoprecipitation - Anti-Histone H2B (acetyl K15) antibody (ab240891)Histone H2B (acetyl K215) was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (treated with 30mM sodium butyrate for 4hr) with ab240891 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Lane 2: ab240891 IP in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr) 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
