Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19800] to Histone H2A (ubiquityl K119) - BSA and Azide free
  • Suitable for: PepArr, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR19800] to Histone H2A (ubiquityl K119) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH/3T3 and C6 whole cell lysates.ICC/IF: HeLa and NIH/3T3 cells.

  • General notes

    Ab240993 is the carrier-free version of ab193203. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19800

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)

    Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)

    Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)
  • Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)
    Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)

    ab193203 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)
  • Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)
    Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (ab240993)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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