Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF-1 alpha antibody - C-terminal (ab228649)

Key features and details

  • Rabbit polyclonal to HIF-1 alpha - C-terminal
  • Suitable for: WB, IP, IHC-P, ChIP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HIF-1 alpha antibody - C-terminal
    See all HIF-1 alpha primary antibodies

  • Description

    Rabbit polyclonal to HIF-1 alpha - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human HIF-1 alpha (C terminal). The exact sequence is proprietary.
    Database link: Q16665

  • Positive control

    • ICC/IF: CoCl2 treated HeLa and NIH/3T3 cells. WB: O2 treated HepG2 and MCF7 whole cell extracts; CoCL2 treated HepG2, NIH/3T3 and HeLa whole cell extracts.; HIF-1 alpha transfected HEK-293T whole cell extract. IHC-P: Human kidney cancer tissue. ChIP: HepG2 chromatin extract. IP: CoCl2 treated HepG2 whole cell extract.

Properties

Images

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 500 µM CoCl2 for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF-1 alpha (green) using ab228649 at 1/500 dilution in ICC/IF.

    Counterstain: Phalloidin, a cytoskeleton marker, at 1/100 dilution (red).

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/500 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) treated with 500 µM CoCl2 for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Flag-tagged HIF-1 alpha, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • ChIP - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    ChIP - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either normal rabbit IgG or ab228649. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 1% O2 for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    HIF-1 alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 μM CoCl2 for 24 hours, whole cell extract using 5 µg of ab228649. Western blot was performed from the immunoprecipitate using ab228649.

    Lane 1: Control IgG IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.
    Lane 2: ab228649 IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    4% paraformaldehyde-fixed, treated and mock-treated NIH/3T3 (mouse embryo fibroblast cell line) cells stained for HIF-1 alpha (green) using ab228649 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/5000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 200 µM CoCl2 for 24 hours, whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 µM CoCl2 for 24 hours, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    Paraffin-embedded human kidney cancer tissue stained for HIF-1 alpha using ab228649 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    All lanes : Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution

    Lane 1 : Non-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lanes 2-3 & 5 : HIF-1 alpha shRNA-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 4 : HIF-1 alpha shRNA-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extracts
    Lane 6 : Non-transfected MCF7 (human breast adenocarcinoma cell line) treated with 1% O2, whole cell extract
    Lanes 7-10 : HIF-1 alpha shRNA-transfected MCF7 (human breast adenocarcinoma cell line) treated with 1% O2, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 93 kDa



    7.5% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)

    4% paraformaldehyde-fixed untreated (left panel) and 500 μM CoCl2 treated (right panel) HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF-1 alpha (green) using ab228649 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Phalloidin, a cytoskeleton marker, at 1/100 dilution (red).

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