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Signal Transduction Metabolism Amino Acids

Anti-HGD antibody [EPR7874] (ab131035)

Price and availability

234 528 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-HGD antibody [EPR7874] (ab131035)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7874] to HGD
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-HGD antibody [EPR7874]
    See all HGD primary antibodies
  • Description

    Rabbit monoclonal [EPR7874] to HGD
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human HGD aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Caco-2, HepG2, and Human fetal liver lysates; Human kidney tissue; HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7874
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Western blot - Anti-HGD antibody [EPR7874] (ab131035)
    Western blot - Anti-HGD antibody [EPR7874] (ab131035)
    All lanes : Anti-HGD antibody [EPR7874] (ab131035) at 1/1000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (ab131035)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (ab131035)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HGD with ab131035 antibody at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874] (ab131035)
    Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874] (ab131035)
    Immunofluorescence analysis of HepG2 cells labelling HGD with ab131035 antibody at 1/100 dilution.
  • Anti-HGD antibody [EPR7874] (ab131035)
    Anti-HGD antibody [EPR7874] (ab131035)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HGD antibody [EPR7874] (ab131035)

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    Applications: ICC/IF, IHC-P

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    Anti-HGD antibody (ab231968)

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    Anti-HGD antibody (ab225926)

    Applications: IHC-P, WB

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