Asparaginase Activity Assay Kit (ab107922)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Assay time: 40 min
- Sample type: Cell Lysate
Overview
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Product name
Asparaginase Activity Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate -
Assay type
Enzyme activity -
Assay time
0h 40m -
Product overview
Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.
In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
Asparaginase assay protocol summary:
- add reaction mix to sample and standard wells, mix and incubate for 10 min
- analyze with microplate reader in kinetic mode for 30-60 min -
Notes
Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Storage buffer
Preservative: None
Constituents: DMSO -
Components Identifier 100 tests Asparaginase Assay Buffer WM 1 x 25ml Aspartate Enzyme Mix (lyophilized) Green 1 vial Aspartate Standard (100 mM) Yellow 1 x 0.1ml Conversion Mix (lyophilized) Purple 1 vial Positive Control (lyophilized) Blue 1 vial Probe (in DMSO) Red 1 x 0.2ml Substrate Mix (lyophilized) Orange 1 vial -
Research areas
Images
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Functional Studies - Asparaginase Activity Assay Kit (Colorimetric)
Typical asparaginase standard calibration curve using colorimetric reading.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Typical asparaginase standard calibration curve using fluorometric reading.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.