Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)
![Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)](https://www.abcam.com/ps/products/8/ab8637/Images/ab8637_1.jpg "Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)")
Key features and details
- Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
- Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IF, IHC-P
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
Overview
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Product name
Anti-Hepatitis B Virus Core Antigen antibody [C1]
See all Hepatitis B Virus Core Antigen primary antibodies -
Description
Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen -
Host species
Mouse -
Specificity
This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
Ab8637 was raised against serotype ayw but will work with all other genotypes.
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Tested applications
Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Purified Hepatitis B Core Antigen
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Epitope
Around aa positions 70-80, major, or "c1" determinant -
General notes
This product was raised against native assembled HBV core (HBV capsid, or particulated core).
For denaturing western blots and denaturing Immunofluorescence we would recommend one of the following:
Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639)
Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)
These products are raised to the same, but denatured, HBcAg protein sequence thus have more robust activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Ascitic fluid -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
C1 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)
Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183
Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183