Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Hemojuvelin antibody [C5] (ab239497)

Key features and details

Mouse monoclonal [C5] to Hemojuvelin
Suitable for: WB, IHC-P
Reacts with: Rat, Human
Isotype: IgG2b

Product name

Anti-Hemojuvelin antibody [C5]
See all Hemojuvelin primary antibodies
Description

Mouse monoclonal [C5] to Hemojuvelin
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Recombinant fragment (His-tag) corresponding to Human Hemojuvelin aa 200 to the C-terminus. N-terminal His-tag (Expressed in E.coli).
Database link: Q6ZVN8

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human kidney, stomach, glioma and liver tissues. WB: Rat liver lysate; Recombinant human Hemojuvelin protein.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

C5
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [C5] (ab239497)

Paraffin-embedded human kidney tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Western blot - Anti-Hemojuvelin antibody [C5] (ab239497)

Anti-Hemojuvelin antibody [C5] (ab239497) at 2 µg/ml + Rat liver lysate

Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [C5] (ab239497)

Paraffin-embedded human stomach tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [C5] (ab239497)

Paraffin-embedded human glioma tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [C5] (ab239497)

Paraffin-embedded human liver tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Western blot - Anti-Hemojuvelin antibody [C5] (ab239497)

Anti-Hemojuvelin antibody [C5] (ab239497) at 2 µg/ml + Recombinant human Hemojuvelin protein

Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 45 kDa

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