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Neuroscience Neurology process Notch Pathway

Anti-GSK3 beta antibody (ab227208)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GSK3 beta antibody (ab227208)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GSK3 beta
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GSK3 beta antibody
    See all GSK3 beta primary antibodies
  • Description

    Rabbit polyclonal to GSK3 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Pig
  • Immunogen

    Recombinant fragment within Human GSK3 beta (internal sequence). The exact sequence is proprietary.
    Database link: P49841

  • Positive control

    • WB: HEK-293T and K562 whole cell extracts; Mouse brain lysate. IHC-P: H661 xenograft tissue; Rat brain tissue. ICC/IF: SK-N-SH cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-GSK3 beta antibody (ab227208)
    Western blot - Anti-GSK3 beta antibody (ab227208)
    All lanes : Anti-GSK3 beta antibody (ab227208) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : GSK3 beta shRNA transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-GSK3 beta antibody (ab227208)
    Western blot - Anti-GSK3 beta antibody (ab227208)
    Anti-GSK3 beta antibody (ab227208) at 1/3000 dilution + Mouse brain lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-GSK3 beta antibody (ab227208)
    Western blot - Anti-GSK3 beta antibody (ab227208)
    Anti-GSK3 beta antibody (ab227208) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody (ab227208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody (ab227208)

    Paraffin-embedded H661 (human) xenograft tissue stained for GSK3 beta using ab227208 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody (ab227208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody (ab227208)

    Paraffin-embedded rat brain tissue stained for GSK3 beta using ab227208 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta antibody (ab227208)
    Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta antibody (ab227208)

    4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for GSK3 beta (green) using ab227208 at 1/400 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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