All lanes : Anti-GSK3 beta antibody [3D10] (ab93926) at 1/500 dilution

Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : HEK293 cell lysate

Predicted band size: 47 kDa

ab93926, at a 1/4000 dilution, staining GSK3 beta in NIH/3T3 cells (left) and U251 cells (right) by Immunofluorescence (green). DRAQ5 fluorescent DNA dye is blue and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

ab93926, at a 1/4000 dilution, staining GSK3 beta in paraffin embedded Human lung cancer (left) and breast cancer (right) tissues by Immunohistochemistry. Detection: DAB staining.

Overlay histogram showing HeLa cells stained with ab93926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93926, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.