Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
![Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-1-anti-gsdmc2gsdmc3-antibody-epr2089048-immunohistochemistry-mouse-stomach.jpg "Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3
- Suitable for: IHC-P, IHC-Fr, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48]
See all GSDMC2+GSDMC3 primary antibodies -
Description
Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseWB MouseHuman
-
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: Mouse stomach, small intestine, colon and caecum tissue lysates; HEK-293T transfected with mGSDMC2 expression vector whole cell lysate; HEK-293T transfected with mGSDMC3 expression vector whole cell lysate. IHC-P: Mouse stomach tissue. IHC-Fr: Mouse stomach and colon tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20890-48 -
Isotype
IgG
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplamic staining on epithelial cells in mouse stomach (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-412153-anti-gsdmc2gsdmc3-antibody-epr2089048-western-blot-tissue.jpg)
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : Wild-type mouse esophagus tissue lysate
Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
Lane 3 : Wild-type Mouse stomach tissue lysate
Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
Lane 5 : Wild-type mouse small intestine tissue lysate
Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
Lane 7 : Wild-type mouse colon tissue lysate
Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
Lane 9 : Wild-type mouse caecum tissue lysate
Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
-
![Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-6-anti-gsdmc2gsdmc3-antibody-epr2089048-immunohistochemistry-frozen-mouse-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-412152-anti-gsdmc2gsdmc3-antibody-epr2089048-western-blot-lysate.jpg)
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (PMID: 17350798).
Exposure time: 54 seconds.
-
![Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-5-anti-gsdmc2gsdmc3-antibody-epr2089048-immunohistochemistry-frozen-mouse-stomach.jpg)
Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse stomach is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-412154-anti-gsdmc2gsdmc3-antibody-epr2089048-western-blot-kidney.jpg)
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-2-anti-gsdmc2gsdmc3-antibody-epr2089048-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)](https://www.abcam.com/ps/products/229/ab229896/Images/ab229896-10-benefits-of-recombinant-antibodies.png)
Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)