Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-HDAC4 antibody [EPR22937-157] (ab235583)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22937-157] to HDAC4
Suitable for: WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-HDAC4 antibody [EPR22937-157]
See all HDAC4 primary antibodies
Description

Rabbit monoclonal [EPR22937-157] to HDAC4
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HAP1 whole cell lysate, HEK-293T, HeLa, A549, Neuro-2a, RAW 264.7 and NIH/3T3 lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22937-157
Isotype

IgG
Research areas

Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HDAC4 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 119 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

ab235583 was shown to specifically react with HDAC4 in wild-type HAP1 cells as signal was lost in HDAC4 knockout cells. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE.

ab235583 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc^™ MP instrument using the ECL technique.

Exposure time: 3 minutes.

Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 119 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes.

Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 119 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Lanes 3-4 in this blot were developed using a higher sensitivity ECL substrate.

Exposure time: Lanes 1-2: 81 seconds Lane 3: 48 seconds Lane 4: 15 seconds.

Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
Anti-HDAC4 antibody [EPR22937-157] (ab235583)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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