Anti-HDAC4 antibody [HDAC-144] (ab12171)
![Anti-HDAC4 antibody [HDAC-144] (ab12171)](https://www.abcam.com/ps/products/12/ab12171/Images/ab12171-269784-anti-hdac4-antibody-hdac-144-chip-grade-western-blot.jpg "Anti-HDAC4 antibody [HDAC-144] (ab12171)")
Key features and details
- Mouse monoclonal [HDAC-144] to HDAC4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-HDAC4 antibody [HDAC-144]
See all HDAC4 primary antibodies -
Description
Mouse monoclonal [HDAC-144] to HDAC4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. -
Positive control
- WB: HeLa, NIH/3T3, P19, Jurkat and PC-12 cell lysates; Rat brain lysate. Total cell extracts of NIH3T3 fibroblast cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 23/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HDAC-144 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-HDAC4 antibody [HDAC-144] (ab12171)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: HDAC4 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: NIH3T3 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12171 observed at 140 kDa. Red - loading control, ab176560, observed at 52 kDa.ab12171 was shown to recognize HDAC4 when HDAC4 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE. Ab12171 and ab176560 (loading control to alpha tubulin) were diluted at 1 µg/ml and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
This image was generated using the ascites version of the product.
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![Western blot - Anti-HDAC4 antibody [HDAC-144] (ab12171)](https://www.abcam.com/ps/products/12/ab12171/Images/ab12171-295030-anti-hdac4-antibody-hdac-144-chip-grade-western-blot.jpg)
Western blot - Anti-HDAC4 antibody [HDAC-144] (ab12171)All lanes : Anti-HDAC4 antibody [HDAC-144] (ab12171) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 3 : P19 cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 5 : Rat brain lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 119 kDaThis image was generated using the ascites version of the product.
