Anti-GRK2 antibody (ab235240)
Key features and details
- Goat polyclonal to GRK2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRK2 antibody
See all GRK2 primary antibodies -
Description
Goat polyclonal to GRK2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: GRK2 HEK-293T overexpressing lysate; THP-1 lysate. IHC-P: Human high grade lymphoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human high grade lymphoma tissue stained for GRK2 using ab235240 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. Magnification 20x.
Antigen retrieval: Heat induced epitope retrieval (HIER).
The Abs developed a specific cytoplasmic staining and partially weak nucelear staining in human high grade lymphoma tissue.
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All lanes : Anti-GRK2 antibody (ab235240) at 1/1000 dilution
Lane 2 : GRK2 HEK-293T over expressing lysate.
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) empty vector lysate.
Secondary
All lanes : Dk-a-Goat IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 80 kDa
Exposure time: 120 secondsBlocking/diluting buffer: 5% BLOTTO.
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Anti-GRK2 antibody (ab235240) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate
Secondary
Dk-a-Goat IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 80 kDa
Exposure time: 120 secondsBlocking/Diluting buffer and concentration: 5% BLOTTO.