Anti-GRIP1 antibody (ab226201)
Key features and details
- Rabbit polyclonal to GRIP1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRIP1 antibody
See all GRIP1 primary antibodies -
Description
Rabbit polyclonal to GRIP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human GRIP1 aa 1026-1076. The exact sequence is proprietary. (NP_066973.2).
Database link: Q9Y3R0-3 -
Positive control
- WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226201 was affinity purified using an epitope specific to GRIP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRIP1 antibody (ab226201)All lanes : Anti-GRIP1 antibody (ab226201) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-GRIP1 antibody (ab226201)GRIP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226201 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226201 at 1 µg/ml.
Lane 1: ab226201 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
