Anti-GRIP1 antibody (ab25963)
Key features and details
- Rabbit polyclonal to GRIP1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GRIP1 antibody
See all GRIP1 primary antibodies -
Description
Rabbit polyclonal to GRIP1 -
Host species
Rabbit -
Specificity
This antibody is raised against glutamate receptor-interacting protein 1 -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse GRIP1.
Read Abcam's proprietary immunogen policy (Peptide available as ab27748.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications HumanApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).Abcam recommends using BSA as the blocking agent.
Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).Abcam recommends using BSA as the blocking agent.
Target
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Function
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons. -
Sequence similarities
Contains 7 PDZ (DHR) domains. -
Domain
PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR. -
Cellular localization
Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 23426 Human
- Entrez Gene: 74053 Mouse
- Entrez Gene: 84016 Rat
- Omim: 604597 Human
- SwissProt: Q9Y3R0 Human
- SwissProt: Q925T6 Mouse
- SwissProt: P97879 Rat
- Unigene: 505946 Human
see all -
Alternative names
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
see all
Images
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Western blot - Anti-GRIP1 antibody (ab25963)All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
References (2)
ab25963 has been referenced in 2 publications.
- Barnes SJ et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron 96:871-882.e5 (2017). IHC-P . PubMed: 29107520
- Gainey MA et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A 112:E3590-9 (2015). PubMed: 26109571
Images
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Western blot - Anti-GRIP1 antibody (ab25963)All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
