Anti-Granuphilin antibody (ab224047)
Key features and details
- Rabbit polyclonal to Granuphilin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Granuphilin antibody
See all Granuphilin primary antibodies -
Description
Rabbit polyclonal to Granuphilin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment corresponding to Human Granuphilin aa 150-350.
Database link: Q96C24 -
Positive control
- IHC: Human pancreas tissue. WB: Granuphilin overexpression HEK-293T lysate. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Granuphilin antibody (ab224047)All lanes : Anti-Granuphilin antibody (ab224047) at 1/250 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate (negative control)
Lane 2 : Granuphilin over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Predicted band size: 76 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (ab224047)Paraffin-embedded human pancreas tissue stained for Granuphilin using ab224047 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Granuphilin antibody (ab224047)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Granuphilin (green) using ab224047 at 4 µg/ml in ICC/IF.
