Anti-Glutaminase C antibody (ab262716)
Key features and details
- Rabbit polyclonal to Glutaminase C
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Glutaminase C antibody
See all Glutaminase C primary antibodies -
Description
Rabbit polyclonal to Glutaminase C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Glutaminase C (C terminal). The exact sequence is proprietary.
Database link: O94925 -
Positive control
- IP: MDA-MB-231 whole cell extracts. IHC-P: Mouse duodenum tissue. Human breast carcinoma and colon carcinoma tissue. ICC/IF: A549 cells. WB: GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Glutaminase C antibody (ab262716)All lanes : Anti-Glutaminase C antibody (ab262716) at 1/5000 dilution
Lane 1 : Non-transfected MDA-MB-231 whole cell extracts
Lane 2 : GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDaThis antibody specifically recognizes GAC but not the KGA isoform.
7.5% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody (ab262716)A549 cells stained for Glutaminase C using ab262716 at a 1/500 dilution (green) in ICC/IF.
Cells were fixed in 4% paraformaldehyde at RT for 15 mins.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody diluted at 1/500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm. -
Immunoprecipitation - Anti-Glutaminase C antibody (ab262716)Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of ab262716.
Western blot analysis was performed using ab262716.
EasyBlot anti-Rabbit IgG was used as a secondary reagent.Lane 1: Input
Lane 2: Control IgG IP
Lane 3: ab262716 IP
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (ab262716)Paraffin embedded mouse duodenum tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (ab262716)Paraffin embedded human colon carcinoma tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (ab262716)Paraffin embedded human breast carcinoma tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15mins.
