Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate receptor 4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate receptor 4 antibody [EPR24283-69] - BSA and Azide free
    See all Glutamate receptor 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat cerebellum and brain; Mouse cerebellum and brain; Human cerebellum tissue lysates. IHC-P: Mouse cerebrum; Rat cerebrum; Human cerebrum.

  • General notes

    ab280206 is the carrier-free version of ab280199. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate at 20 µg
    Lane 2 : Human liver tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 102 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280199, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

    Negative control: human liver (PMID:1372042)

    Exposure time: 3.25 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

    This data was developed using ab280199, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

    Lane 1 : Rat cerebellum tissue lysate at 20 µg
    Lane 2 : Rat brain tissue lysate at 20 µg
    Lane 3 : Rat liver tissue lysate at 40 µg
    Lane 4 : Mouse cerebellum tissue lysate at 20 µg
    Lane 5 : Mouse brain tissue lysate at 20 µg
    Lane 6 : Mouse liver tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 102 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    This data was developed using ab280199, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

    Negative control:  liver (PMID:1372042)

    Exposure time: 7.75 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

    This data was developed using ab280199, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. (PMID: 20662939) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

    This data was developed using ab280199, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

    This data was developed using ab280199, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. (PMID: 1372042) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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