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Anti-GFAP antibody [2A5] (ab4648)

Key features and details

  • Mouse monoclonal [2A5] to GFAP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG1

Overview

  • Product name

    Anti-GFAP antibody [2A5]
    See all GFAP primary antibodies

  • Description

    Mouse monoclonal [2A5] to GFAP

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IHC-FrFl
    Rat
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    Pig
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • Positive control

    • IHC-P: Human brain tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.

  • General notes

    This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.

Properties

Images

  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

    Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

     

  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution

    Lane 1 : MW markers
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Pig brain tissue lysate
    Lane 7 : Recombinant rat GFAP protein
    Lane 8 : Recombinant human GFAP protein

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

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