Anti-GFAP antibody [2A5] (ab4648)
![Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-402154-anti-gfap-antibody-if-rat-cerebellum-tissue.JPG "Anti-GFAP antibody [2A5] (ab4648)")
Key features and details
- Mouse monoclonal [2A5] to GFAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-GFAP antibody [2A5]
See all GFAP primary antibodies -
Description
Mouse monoclonal [2A5] to GFAP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-FrFl RatIHC-P HumanWB MouseRatHumanPig
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Immunogen
Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
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Positive control
- IHC-P: Human brain tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
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General notes
This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentrated Tissue Culture Supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
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![Western blot - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-402153-anti-gfap-antibody-wb-rat-and-mouse-samples.JPG)
Western blot - Anti-GFAP antibody [2A5] (ab4648)Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
Lane 1 : MW markers
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Recombinant rat GFAP protein
Lane 8 : Recombinant human GFAP protein
Predicted band size: 49 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9395-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9393-anti-gfap-antibody-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9397-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera. -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9396-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red). -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9394-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
