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Mouse Nestin ELISA Kit (ab223859)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 7.6 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Nestin ELISA Kit
    See all Nestin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 14.1%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    7.6 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 107 103% - 112%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Nestin ELISA Kit (ab223859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nestin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nestin with 7.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Nestin is an intermediate filament protein and is a marker for neural stem cells.  Nestin is required for brain and eye development.   Nestin expression generally doesn’t persist into adult hood, with the exception of the neuronal precursor cells of the subgranular zone.  After cells have differentiated Nestin is downregulated and is replaced by tissue-specific intermediate filament proteins.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse Nestin ELISA Kit (ab223859)
    Other - Mouse Nestin ELISA Kit (ab223859)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTR
    Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract load
    Interpolated concentrations of native Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract load

    The concentrations of Nestin were measured in duplicate and interpolated from the Nestin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nestin concentration was determined to be 678 pg/mL in NIH/3T3 Cell extract.

  • Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)
    Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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