Anti-GEF H1 antibody (ab155785)
Key features and details
- Rabbit polyclonal to GEF H1
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GEF H1 antibody
See all GEF H1 primary antibodies -
Description
Rabbit polyclonal to GEF H1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 656-1000 of Human GEF H1.
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Positive control
- 293T, A431, HeLa, hMSC-3A6, RAW264.7 and C2C12 whole cell lysates; GEF H1 protein; HeLa cells; Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GEF H1 antibody (ab155785)All lanes : Anti-GEF H1 antibody (ab155785) at 1/2000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 111 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (ab155785) Image is courtesy of an AbReview submitted by Dr Armen Petrosyan.Immunocytochemical immunofluorescence analysis of formaldehyde-fixed human HepG2 cells, labelling GEF H1 with ab155785 with a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST diluent. Permeablization with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST incubated for 1 hour at 22°C. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 594 at 1/200. Counterstain is DAPI (blue) against nuclear DNA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (ab155785)Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution. -
Immunoprecipitation - Anti-GEF H1 antibody (ab155785)Immunoprecipitation of GEF H1 from 293T whole cell extracts using 5 μg of ab155785. Western blot analysis was performed using ab155785.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GEF H1 antibody (ab155785)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
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Western blot - Anti-GEF H1 antibody (ab155785)All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1 : hMSC-3A6 whole cell lysate, mock transfected
Lane 2 : hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNA
Lysates/proteins at 10 µg per lane.
Predicted band size: 111 kDa
8% SDS PAGE -
Western blot - Anti-GEF H1 antibody (ab155785)All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1 : RAW264.7 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 111 kDa
5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GEF H1 antibody (ab155785)Immunofluorescent analysis of methanol-fixed (-20°C 100% MeOH for 5 min) HeLa cells, labeling GEF H1 with ab155785 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
