Anti-GEF H1 (phospho S885) antibody (ab74156)
Key features and details
- Rabbit polyclonal to GEF H1 (phospho S885)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GEF H1 (phospho S885) antibody
See all GEF H1 primary antibodies -
Description
Rabbit polyclonal to GEF H1 (phospho S885) -
Host species
Rabbit -
Specificity
ab74156 detects endogenous levels of GEF H1 only when phosphorylated at serine 885. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human GEF H1 around the phosphorylation site of serine 885 (R-R-SP-L-P).
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Positive control
- Extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin (40nM, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74156 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GEF H1 (phospho S885) antibody (ab74156)All lanes : Anti-GEF H1 (phospho S885) antibody (ab74156) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with TSA (400nM, 24hours)
Lane 2 : Extracts from Jurkat cells treated with
forskolin (40nM, 30mins)
Lane 3 : Extracts from HeLa cells treated
with TSA (400nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
