Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF 9 antibody [EPR22495-129] (ab254323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22495-129] to GDF 9
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GDF 9 antibody [EPR22495-129]
    See all GDF 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR22495-129] to GDF 9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat ovary tissue. IHC-Fr: Mouse ovary tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling GDF 9 with ab254323 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Immunohistochemistry (Frozen sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse ovary tissue labeling GDF 9 with ab254323 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on follicles of mouse ovary (PMID: 21401961) is observed. Counterstained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 22732078). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-GDF 9 antibody [EPR22495-129] (ab254323)
    Anti-GDF 9 antibody [EPR22495-129] (ab254323)

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