Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11326(B)] to ATP6V1D
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-ATP6V1D antibody [EPR11326(B)]
See all ATP6V1D primary antibodies
Description

Rabbit monoclonal [EPR11326(B)] to ATP6V1D
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide, corresponding to residues in Human ATP6V1D (Uniprot: Q9Y5K8).
Positive control
- Human breast and kidney tissues; Human fetal brain, Y79, 293T and HeLa whole cell lysate (ab150035); HeLa and 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11326(B)
Isotype

IgG
Research areas

Western blot - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green).
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution.
Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.

Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution + Immunoprecipitation pellet from Y79 cell lysate at 10 µg
Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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