Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11326(B)] to ATP6V1D
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ATP6V1D antibody [EPR11326(B)]
See all ATP6V1D primary antibodies -
Description
Rabbit monoclonal [EPR11326(B)] to ATP6V1D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide, corresponding to residues in Human ATP6V1D (Uniprot: Q9Y5K8).
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Positive control
- Human breast and kidney tissues; Human fetal brain, Y79, 293T and HeLa whole cell lysate (ab150035); HeLa and 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11326(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
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Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green).
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Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution.
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Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.
Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution + Immunoprecipitation pellet from Y79 cell lysate at 10 µg
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