Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Adherent cells, Cell culture media, Other biological fluids, Plasma, Serum, Suspension cells, Tissue Extracts, Urine
- Sensitivity: 1 uU/ml
Overview
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Product name
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
See all Lactate Dehydrogenase kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
1 uU/ml -
Assay time
0h 20m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).
This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min -
Notes
Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 500 tests LDH Assay Buffer 1 x 110ml LDH Substrate Mix 1 vial NADH Standard Yellow 1 vial PicoProbe Blue 1 x 1.4ml Positive Control 1 vial -
Research areas
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
Database links
- SwissProt: Q7SI97 Other species
Images
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Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
Typical NADH Standard Curve obtained following assay protocol.
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Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.
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Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).
