Anti-GCNA1 antibody [TRA98] (ab82527)
![Anti-GCNA1 antibody [TRA98] (ab82527)](https://www.abcam.com/ps/products/82/ab82527/Images/ab82527-261821-ab82527.jpg "Anti-GCNA1 antibody [TRA98] (ab82527)")
Key features and details
- Rat monoclonal [TRA98] to GCNA1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-GCNA1 antibody [TRA98] -
Description
Rat monoclonal [TRA98] to GCNA1 -
Host species
Rat -
Specificity
In PubMed: 27718356 the epitope of ab82527 has been mapped to a murine-specific 8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat that occurs 25 times in mouse GCNA1 (aa 167- 383). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse GCNA1. Adult mouse testis cell lysates
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Positive control
- Testicular germ cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purification notes
This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions. -
Clonality
Monoclonal -
Clone number
TRA98 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-GCNA1 antibody [TRA98] (ab82527) This image is courtesy of an Abreview by Bryan Niedenberger.
ab82527 staining GCNA1 in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150165 was used as the secondary antibody, undiluted.
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![Western blot - Anti-GCNA1 antibody [TRA98] (ab82527)](https://www.abcam.com/ps/products/82/ab82527/Images/JLP-Primary-antibodies-ab82527-1.JPG)
Western blot - Anti-GCNA1 antibody [TRA98] (ab82527)All lanes : Anti-GCNA1 antibody [TRA98] (ab82527) at 0.1 µg/ml
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
Lane 3 : mouse intestine lysate
Lane 4 : mouse kidney lysate
Lane 5 : mouse liver lysate
Lane 6 : mouse lung lysate
Lane 7 : mouse spleen lysate
Lane 8 : mouse ovary lysate
Lane 9 : mouse testis lysate
Lysates/proteins at 100 µg per lane.
Predicted band size: 146 kDa
Western blot analysis of various mouse tissues, using ab82527.
In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.