Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
![Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)](https://www.abcam.com/ps/products/248/ab248137/Images/ab248137-353159-antigclmantibodyepr6667westernblot.jpg "Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6667] to GCLM - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GCLM antibody [EPR6667] - BSA and Azide free
See all GCLM primary antibodies -
Description
Rabbit monoclonal [EPR6667] to GCLM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa cell lysate.
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General notes
Ab248137 is the carrier-free version of ab126704. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6667 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GCLM knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 31 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126704).
Lanes 1 - 3: Merged signal (red and green). Green - ab126704 observed at 31 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab126704 was shown to recognize GCLM in wild-type HAP1 cells as signal was lost at the expected MW in GCLM knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GCLM knockout samples were subjected to SDS-PAGE. Ab126704 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)](https://www.abcam.com/ps/products/248/ab248137/Images/ab248137-5-benefits-of-recombinant-antibodies.png)
Anti-GCLM antibody [EPR6667] - BSA and Azide free (ab248137)
