Anti-GCH1 antibody [OTI5A1] (ab236387)
![Anti-GCH1 antibody [OTI5A1] (ab236387)](https://www.abcam.com/ps/products/236/ab236387/Images/ab236387-312320-anti-gtp-cyclohydrolase-1-antibody-oti5a1-western-blot.jpg "Anti-GCH1 antibody [OTI5A1] (ab236387)")
Key features and details
- Mouse monoclonal [OTI5A1] to GCH1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-GCH1 antibody [OTI5A1]
See all GCH1 primary antibodies -
Description
Mouse monoclonal [OTI5A1] to GCH1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
-
Immunogen
Recombinant full length protein corresponding to Human GCH1 aa 1 to the C-terminus. Produced in E.coli (NP_001019195).
Database link: P30793-1 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GCH1 cDNA for 48 hrs. IHC-P: Human skin, placenta and spleen carcinoma tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5A1 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-GCH1 antibody [OTI5A1] (ab236387)All lanes : Anti-GCH1 antibody [OTI5A1] (ab236387) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GCH1 cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 28 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)](https://www.abcam.com/ps/products/236/ab236387/Images/ab236387-312319-anti-gtp-cyclohydrolase-1-antibody-oti5a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)Paraffin-embedded human skin tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)](https://www.abcam.com/ps/products/236/ab236387/Images/ab236387-312317-anti-gtp-cyclohydrolase-1-antibody-oti5a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)Paraffin-embedded human placenta tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)](https://www.abcam.com/ps/products/236/ab236387/Images/ab236387-312315-anti-gtp-cyclohydrolase-1-antibody-oti5a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)Paraffin-embedded human spleen carcinoma tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.
