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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins

Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)

Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14778] to Gbeta5 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free
    See all Gbeta5 primary antibodies
  • Description

    Rabbit monoclonal [EPR14778] to Gbeta5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Rat
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250806 is the carrier-free version of ab185206. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250806 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14778
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins

Images

  • Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/2000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab185206, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)

    This data was developed using ab185206, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)

    This data was developed using ab185206, the same antibody clone in a different buffer formulation.

    Western blot analysis of Gbeta5 in immunoprecipitation pellets from Rat brain lysate using ab185206 at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
  • Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    This data was developed using ab185206, the same antibody clone in a different buffer formulation.Flow cytometry analysis of 2% paraformaldehyde fixed HACAT cells labeling Gbeta5 with ab185206 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
  • Immunocytochemistry - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Immunocytochemistry - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    This data was developed using ab185206, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed U87-MG cells labeling Gbeta5 with ab185206 at 1/100 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstained with Dapi blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    This data was developed using ab185206, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab185206, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Western blot - Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab185206, the same antibody clone in a different buffer formulation.

     

  • Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)
    Anti-Gbeta5 antibody [EPR14778] - BSA and Azide free (ab250806)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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