Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23931-27] to GAS 6 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free
    See all GAS 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23931-27] to GAS 6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, SH-SY5Y and RPMI-8226 whole cell lysates Flow Cyt: THP-1 and THP-1 cells. IP: THP-1 whole cell lysate.

  • General notes

    ab280361 is the carrier-free version of ab264098. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280361 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

    This data was developed using ab264098, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098

  • Western blot - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Western blot - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    All lanes : Anti-GAS 6 antibody [EPR23931-27] (ab264098) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : Jurkat (human t cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 4 : RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 75 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab264098, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Jurkat (PMID:10400186)

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lanes 1-2: 48 seconds;Lanes 3-4: 3 minutes.

  • Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

    This data was developed using ab264098, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098. Cells were co-stained with DRAQ/ Ki-67 to differentiate cell cycle phase.

  • Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

    This data was developed using ab264098, the same antibody clone in a different buffer formulation.

    GAS 6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab264098 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264098 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 10 ug

    Lane 2: ab264098 IP in THP-1 whole cell lysate

    Lane 3: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated: Rabbit monoclonal IgG (ab172730) instead of ab264098 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

    This data was developed using ab264098, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (Human T cell leukemia T lymphocyte (Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID:10400186)

  • Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)
    Anti-GAS 6 antibody [EPR23931-27] - BSA and Azide free (ab280361)

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