Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-gamma Synuclein/SNCG antibody [EP1539Y]
    See all gamma Synuclein/SNCG primary antibodies

  • Description

    Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within gamma Synuclein/SNCG aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and SK-BR-3 cell lysates; Human fetal brain lysate. IHC-P: Human brain.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    All lanes : Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SNCG knockout HeLa cell lysate
    Lane 3 : SK-BR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 13 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab52633 observed at 16 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab52633 Anti-gamma Synuclein/SNCG antibody [EP1539Y]  was shown to specifically react with gamma Synuclein/SNCG antibody in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265623 (knockout cell lysate ab257694) was used.  Wild-type and gamma Synuclein/SNCG knockout samples were subjected to SDS-PAGE.  ab52633 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/2000 dilution + Human fetal brain lysate

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

    ab52633 staining human brain in IHC-P.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
    Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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