Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 09, 2021

Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-gamma Synuclein/SNCG antibody [EP1539Y]
See all gamma Synuclein/SNCG primary antibodies
Description

Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within gamma Synuclein/SNCG aa 100-200. The exact sequence is proprietary.
Positive control
- WB: HeLa and SK-BR-3 cell lysates; Human fetal brain lysate. IHC-P: Human brain.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1539Y
Isotype

IgG
Research areas

Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

All lanes : Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNCG knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 13 kDa

Lanes 1-3: Merged signal (red and green). Green - ab52633 observed at 16 kDa. Red - loading control ab8245 observed at 36 kDa.

ab52633 Anti-gamma Synuclein/SNCG antibody [EP1539Y] was shown to specifically react with gamma Synuclein/SNCG antibody in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265623 (knockout cell lysate ab257694) was used. Wild-type and gamma Synuclein/SNCG knockout samples were subjected to SDS-PAGE. ab52633 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/2000 dilution + Human fetal brain lysate

Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

ab52633 staining human brain in IHC-P.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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