Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3
Suitable for: ICC/IF, Flow Cyt, WB, IP
Reacts with: Human

Product name

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]
See all Pentraxin 3/PTX3 primary antibodies
Description

Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab85335)
Positive control
- WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow cytometry: HUVEC cells. IP: HUVEC whole cell lysate.
General notes
Previously labelled as Pentraxin 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18678-105
Isotype

IgG
Research areas

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution

Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).

-ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM/TBST

Higher concentration of this antibody is required for the detection of Pentraxin 3/PTX3 in untreated cells in WB.
Flow Cytometry - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: HUVEC whole cell lysate 10 μg (Input).
Lane 2: ab190838 IP in HUVEC whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

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