Anti-CD163L1 antibody [EPR6539] (ab126756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6539] to CD163L1
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD163L1 antibody [EPR6539]
See all CD163L1 primary antibodies -
Description
Rabbit monoclonal [EPR6539] to CD163L1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Synthetic peptide within Human CD163L1 aa 1400 to the C-terminus (intracellular). The exact sequence is proprietary.
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Positive control
- Human spleen and uterus tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6539 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)Immunohistochemical staining of paraffin embedded human stomach with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)Immunohistochemical staining of paraffin embedded human spleen with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human spleen tissue by Immunohistochemsitry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human uterus tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756 showing positive staining in Normal tonsil macrophages tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756 showing positive staining in Normal liver macrophages tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756 showing positive staining in Normal lung macrophages tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756 showing negative staining in Normal placenta tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (ab126756)
Unpurified ab126756 showing positive staining in Normal stomach macrophages tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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