Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17862-7] to gamma Sarcoglycan
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-gamma Sarcoglycan antibody [EPR17862-7]
    See all gamma Sarcoglycan primary antibodies

  • Description

    Rabbit monoclonal [EPR17862-7] to gamma Sarcoglycan

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal kidney, Human fetal spleen, Mouse muscle or Mouse heart whole cell lysate. IHC-P: Human skeletal muscle. IP: Human fetal kidney whole cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17862-7

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.

     

    Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/10000 dilution + Human skeletal muscle whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer

    5% NFDM/TBST.

     

    This protein is expressed in skeletal and heart muscle.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/1000 dilution + Mouse muscle lysate at 10 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution + Mouse heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 10 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer 

    5% NFDM/TBST.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

    Lane 1 : Human fetal brain whole cell lysate
    Lane 2 : Human fetal heart whole cell lysate
    Lane 3 : Human fetal kidney whole cell lysate
    Lane 4 : Human fetal spleen whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 15 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

    This protein is expressed in skeletal and heart muscle.

    ab181602 Anti-GAPDH antibody [EPR16891] control used.

  • Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

    Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
    Lane 1: Human fetal kidney whole cell lysate 10 µg (Input). Lane 2: ab203113 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 Seconds

     

    Blocking/Dilution buffer 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.

     

    Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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